5 datasets found
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Federal
Complex splicing pattern generates great diversity in human
U.S. Department of Health & Human Services —
Background Mutation analysis of the neurofibromatosis type 1 (NF1) gene has shown that about 30% of NF1 patients carry a splice mutation resulting in the production... -
Federal
Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines
U.S. Department of Health & Human Services —
Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the... -
Federal
Stable hepatitis C virus RNA detection by RT-PCR during four days storage
U.S. Department of Health & Human Services —
Background Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration... -
Federal
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
U.S. Department of Health & Human Services —
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The... -
Federal
A role for the collagen I/III and MMP-1/-13 genes in primary inguinal hernia?
U.S. Department of Health & Human Services —
Background Abnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered...
5 datasets found