{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["026:00"], "contactPoint": {"@type": "vcard:Contact", "fn": "Open Science Data Repository Help Desk", "hasEmail": "mailto:arc-dl-osdr-help@mail.nasa.gov"}, "description": "In the Rodent Research Reference Mission (RRRM-2), forty female C57BL/6NTac mice were flown on the International Space Station. To assess differences in outcomes due to age, twenty 12 week-old and twenty 29 week-old mice were flown, respectively. To directly assess spaceflight effects, half of the young and old mice (10 old, 10 young) were sacrificed on-orbit after 55-58 days (ISS Terminal, ISS-T), while the other half (10 old, 10 young) were returned live to Earth after 32 days and allowed to recover for 24 days (Live Animal Return, LAR) before sacrifice. ISS-T and LAR mice were the same age at sacrifice. Both the ISS-T and LAR animals had independent ground controls (10 mice per group housed in flight hardware in matched environmental conditions), basal controls (10 mice per group sacrificed 2 days before launch), and vivarium controls (10 mice per group housed within standard vivarium habitats). Thus RRRM-2 included a total of 160 mice. This study includes bulk RNA sequencing and single nuclei transcriptomics and epigenomics from left cerebral hemispheres from 4 young ISS-T spaceflight animals, 5 old ISS-T spaceflight animals, 5 young ISS-T ground control animals, and 4 young ISS-T ground control animals.", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://nlsp.nasa.gov/view/lsdapub/lsda_experiment/46969989-6526-5a0f-8829-bed4bff9d331", "format": "BIN", "mediaType": "application/octet-stream"}, {"@type": "dcat:Distribution", "downloadURL": "https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-18", "format": "BIN", "mediaType": "application/octet-stream"}, {"@type": "dcat:Distribution", "downloadURL": "https://osdr.nasa.gov/bio/repo/data/studies/OSD-613", "format": "BIN", "mediaType": "application/octet-stream"}], "identifier": "10.26030/cevt-ja62", "keyword": ["__"], "license": "https://www.usa.gov/government-works", "modified": "2026-04-06", "programCode": ["026:000"], "publisher": {"@type": "org:Organization", "name": "Open Science Data Repository"}, "theme": ["Biological and Physical Sciences"], "title": "Bulk RNA sequencing and single nuclei transcriptomics and epigenomics of brain tissue from mice flown on the RRRM-2 mission"}