{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          The serum response element (SRE) in the c-fos promoter is a convergence point for several signaling pathways that regulate induction of the c-fos gene. Many transcription factors regulate the SRE, including serum response factor (SRF), ternary complex factor (TCF), and CCAAT/enhancer binding protein-beta (C/EBP\u03b2). Independently, the TCFs and C/EBP\u03b2 have been shown to interact with SRF and to respond to Ras-dependent signaling pathways that result in transactivation of the SRE. Due to these common observations, we addressed the possibility that C/EBP\u03b2 and Elk-1 could both be necessary for Ras-stimulated transactivation of the SRE.\n        \n        \n          Results\n          In this report, we demonstrate that Elk-1 and C/EBP\u03b2 functionally synergize in transactivation of both a Gal4 reporter plasmid in concert with Gal4-SRF and in transactivation of the SRE. Interestingly, this synergy is only observed upon activation of Ras-dependent signaling pathways. Furthermore, we show that Elk-1 and C/EBP\u03b2 could interact both in an in vitro GST-pulldown assay and in an in vivo co-immunoprecipitation assay. The in vivo interaction between the two proteins is dependent on the presence of activated Ras. We have also shown that the C-terminal domain of C/EBP\u03b2 and the N-terminal domain of Elk-1 are necessary for the proteins to interact.\n        \n        \n          Conclusions\n          These data show that C/EBP\u03b2 and Elk-1 synergize in SRF dependent transcription of both a Gal-4 reporter and the SRE. This suggests that SRF, TCF, and C/EBP\u03b2 are all necessary for maximal induction of the c-fos SRE in response to mitogenic signaling by Ras.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC29063/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/6qfa-qj53", "issued": "2025-07-13", "keyword": ["nih", "transcription-factors", "serum-response-element", "ras-signaling", "gene-transactivation"], "landingPage": "https://healthdata.gov/d/6qfa-qj53", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "C/EBPBeta and Elk-1 synergistically transactivate the"}