{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Soy products contain isoflavones (genistein, daidzein, and\nglycitein) that display biological effects when ingested by\nhumans and animals, these effects are species, dose and age\ndependent. Therefore, the content and quality of isoflavones in\nsoybeans is a key to their biological effect. Our objective was\nto identify loci that underlie isoflavone content in soybean\nseeds. The study involved 100 recombinant inbred lines (RIL) from\nthe cross of \u2018Essex' by \u2018Forrest,' two cultivars that contrast\nfor isoflavone content. Isoflavone content of seeds from each RIL\nwas determined by high performance liquid chromatography (HPLC).\nThe distribution of isoflavone content was continuous and\nunimodal. The heritability estimates on a line mean basis were\n79% for daidzein, 22% for genistein, and 88% for glycitein.\nIsoflavone content of soybean seeds was compared against 150\npolymorphic DNA markers in a one-way analysis of variance. Four\ngenomic regions were found to be significantly associated with\nthe isoflavone content of soybean seeds across both locations and\nyears. Molecular linkage group B1 contained a major QTL\nunderlying glycitein content (P = 0.0001, R2 = 50.2%), linkage\ngroup N contained a QTL for glycitein (P = 0.0033, R2 = 11.1%)\nand a QTL for daidzein (P = 0.0023, R2 = 10.3%) and linkage\ngroup A1 contained a QTL for daidzein (P = 0.0081, R2 = 9.6%).\nSelection for these chromosomal regions in a marker assisted\nselection program will allow for the manipulation of amounts and\nprofiles of isoflavones (genistein, daidzein, and glycitein)\ncontent of soybean seeds. In addition, tightly linked markers can\nbe used in map based cloning of genes associated with\nisoflavone content.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC79679/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/g52a-w754", "issued": "2025-07-14", "keyword": ["nih", "soybean-isoflavones", "seed-composition", "genetic-mapping", "hplc-analysis"], "landingPage": "https://healthdata.gov/d/g52a-w754", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Genomic regions that underlie soybean seed\nisoflavone content"}