{"accessLevel": "public", "bureauCode": ["020:00"], "contactPoint": {"fn": "Joshua Harrill", "hasEmail": "mailto:harrill.joshua@epa.gov"}, "description": "We screened 44 chemicals in MCF7 cells in concentration response and generated HTTr data using the TempO-Seq hWTv1 assay. First, we provide an outline of the quality of the HTTr data based on a set of QC metrics we developed for this platform. Second, we evaluate the reproducibility and mechanistic accuracy of the HTTr platform using inter-plate analysis of reference samples and comparison of reference chemical treatment effects with CMAP signatures, respectively. Third, we summarize the concentration-dependent HTTr responses for all 44 chemicals to stratify them in terms of their overall effect on the transcriptome. Fourth, we present a new gene signature based concentration-response analysis that provides potency estimates for perturbation of cellular biology (ie, BPACs). \n\nThis dataset is associated with the following publication:\nHarrill, J., L. Everett, D. Haggard, T. Sheffield, J. Bundy, C. Willis, R. Thomas, I. Shah, and R. Judson. High-Throughput Transcriptomics Platform for Screening Environmental Chemicals.   TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA,  181(1): 68-89, (2021).", "distribution": [{"accessURL": "https://gaftp.epa.gov/COMPTOX/CCTE_Publication_Data/BCTD_Publication_Data/Everett/HTTr_Pilot_FigShare/", "title": "https://gaftp.epa.gov/COMPTOX/CCTE_Publication_Data/BCTD_Publication_Data/Everett/HTTr_Pilot_FigShare/"}], "identifier": "https://doi.org/10.23719/1522614", "keyword": ["transcriptomics", "high-throughput screening", "computational toxicology", "TempO-Seq"], "license": "https://pasteur.epa.gov/license/sciencehub-license.html", "modified": "2020-12-22", "programCode": ["020:000"], "publisher": {"name": "U.S. EPA Office of Research and Development (ORD)", "subOrganizationOf": {"name": "U.S. Environmental Protection Agency", "subOrganizationOf": {"name": "U.S. Government"}}}, "references": ["https://doi.org/10.1093/toxsci/kfab009"], "rights": null, "title": "High-Throughput Transcriptomics Platform for Screening Environmental Chemicals"}