{"accessLevel": "public", "bureauCode": ["010:12"], "contactPoint": {"@type": "vcard:Contact", "fn": "Susan Knowles", "hasEmail": "mailto:sknowles@usgs.gov"}, "description": "During an epidemiologic survey following a mortality event of freshwater mussels in 2018 in the Embarrass River, Wisconsin, USA, we identified a novel microsporidian parasite in the ovary of mucket (Actinonaias ligamentina), plain pocketbook (Lampsilis cardium), and fatmucket (Lampsilis siliquoidea) (Unioinidae).  Histopathology showed round-to-oval microsporidial spores in the cytoplasm of oocytes in 60% (3/5) of mucket, 100% (4/4) of plain pocketbook and 50% (1/2) of fatmucket.  On transmission electron microscopy, mature spores were round to oval, measured 4.13 +/- 0.64 \u00b5m (3.14\u20135.31) long by 2.88 +/-0.37 \u00b5m (2.36\u20133.68) wide.  Spores had a thin electron dense exospore with a spiky coat, a thick electron lucent endospore, diplokaryotic nuclei, a polar vacuole, and 27\u201328 polar filaments arranged in 1 to 3 rows.  Sequencing of the small subunit rRNA produced a 1356 bp sequence most similar to Pseudonosema cristatellae, and phylogenetic analysis grouped it with freshwater Neopereziida.  The morphologic and ultrastructural characteristics did not match those of Pseudonosema sp. and a new genus and species, Hirsutonosema embarrassi n. gen., n. sp., were designated.  Additional studies could evaluate host susceptibility, distribution, seasonality, transmission, and lethal or sub-lethal effects of this parasite to freshwater mussels.", "distribution": [{"@type": "dcat:Distribution", "description": "The metadata original format", "downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.660b1df3d34e4df16bd58c39.xml", "format": "XML", "mediaType": "text/xml", "title": "Original Metadata"}, {"@type": "dcat:Distribution", "accessURL": "https://doi.org/10.5066/P1AQGHWQ", "description": "Landing page for access to the data", "format": "XML", "mediaType": "application/http", "title": "Digital Data"}], "identifier": "http://datainventory.doi.gov/id/dataset/USGS_660b1df3d34e4df16bd58c39", "keyword": ["biota", "microsporidia", "USGS:660b1df3d34e4df16bd58c39", "Hirsutonosema embarrassi n. sp.", "wildlife disease", "Lampsilus cardium", "transmission electron microscopy", "Neopereziida", "unionid", "histopathology"], "modified": "2024-06-05T00:00:00Z", "publisher": {"@type": "org:Organization", "name": "U.S. Geological Survey"}, "spatial": "-88.7985782, 44.7397921, -88.7984012, 44.7398997", "theme": ["geospatial"], "title": "2018 Embarrass River Microsporidia"}