{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          The C-terminal four amino acids (GEEV) of human \u03b11A-adrenergic receptors (ARs) have been reported to interact with the PDZ domain of neuronal nitric oxide synthase (nNOS) in a yeast two-hybrid system. The other two \u03b11-AR subtypes have no sequence homology in this region, raising the possibility of subtype-specific protein-protein interactions.\n        \n        \n          Results\n          We used co-immunoprecipitation and functional approaches with epitope-tagged \u03b11-ARs to examine this interaction and the importance of the C-terminal tail. Following co-transfection of HEK-293 cells with hexahistidine/Flag (HF)-tagged \u03b11A-ARs and nNOS, membranes were solubilized and immunoprecipitated with anti-FLAG affinity resin or anti-nNOS antibodies. Immunoprecipitation of HF\u03b11A-ARs resulted in co-immunoprecipitation of nNOS and vice versa, confirming that these proteins interact. However, nNOS also co-immunoprecipitated with HF\u03b11B- and HF\u03b11D-ARs, suggesting that the interaction is not specific to the \u03b11A subtype. In addition, nNOS co-immunoprecipitated with each of the three HF\u03b11-AR subtypes which had been C-terminally truncated, suggesting that this interaction does not require the C-tails; and with Flag-tagged \u03b21- and \u03b22-ARs. Treatment of PC12 cells expressing HF\u03b11A-ARs with an inhibitor of nitric oxide formation did not alter norepinephrine-mediated activation of mitogen activated protein kinases, suggesting nNOS is not involved in this response.\n        \n        \n          Conclusions\n          These results show that nNOS does interact with full-length \u03b11A-ARs, but that this interaction is not subtype-specific and does not require the C-terminal tail, raising questions about its functional significance.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC128815/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/nj87-y6eh", "issued": "2025-07-14", "keyword": ["nih", "adrenergic-receptors", "nitric-oxide-synthase", "protein-interactions", "co-immunoprecipitation"], "landingPage": "https://healthdata.gov/d/nj87-y6eh", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Interaction of neuronal nitric oxide synthase with alpha"}