{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["009:25"], "contactPoint": {"@type": "vcard:Contact", "fn": "NIH", "hasEmail": "mailto:info@nih.gov"}, "description": "Background\n          A standardised technique using a suction-induced mini-erosion that allows serial sampling of dermal interstitial fluid (IF) for 5 to 6 days has been described. In the present study, we studied permeability changes as a function of time.\n        \n        \n          Methods\n          We examined IF concentrations of total protein concentration and the concentration of insulin (6.6 kDa), prealbumin (55 kDa), albumin (66 kDa), transferrin (80 kDa), IgG (150 kDa) and alpha-2-macroglobulin (720 kDa) as a function of time, using an extraction pressure of 200 mmHg below atmospheric.\n        \n        \n          Results\n          At 0 h after forming the erosion, mean total IF protein content (relative to plasma) was 26 \u00b1 13% (SD). For the individual proteins, the relative mean concentrations were 65 \u00b1 36% for insulin, 48 \u00b1 12% for albumin, 30 \u00b1 19% for transferrin, 31 \u00b1 15%for IgG and 19.5 \u00b1 10% for alpha-2-macroglobulin. At 24 h, the total IF protein content was higher than at 0 h (56 \u00b1 26% vs 26 \u00b1 13%; p < 0.05, diff: 115%), as were some of the individual protein concentrations: prealbumin (50 \u00b1 24 vs 25 \u00b1 13%; p < 0.05), albumin (68 \u00b1 21 vs 48 \u00b1 12%; p < 0.05) and IgG (55 \u00b1 30 vs 31 \u00b1 15%; p = 0.05). ln the interval 24 h to 96 h the concentrations were relatively unchanged.\n        \n        \n          Conclusions\n          The results indicate that fluid sampled at 0 h after forming the erosion represents dermal IF before the full onset of inflammation. From 24 h onward, the sampled fluid reflects a steady state of increased permeability induced by inflammation. This technique is promising as a tool for clinically sampling substances that are freely distributed in the body and as a model for studying inflammation and vascular permeability.", "distribution": [{"@type": "dcat:Distribution", "description": "Visit the original government dataset for complete information, documentation, and data access.", "downloadURL": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC77420/", "mediaType": "text/html", "title": "Official Government Data Source"}], "identifier": "https://healthdata.gov/api/views/snc5-rbt6", "issued": "2025-07-14", "keyword": ["nih", "plasma-proteins", "skin-permeability", "interstitial-fluid", "protein-concentration"], "landingPage": "https://healthdata.gov/d/snc5-rbt6", "modified": "2025-09-06", "programCode": ["009:033"], "publisher": {"@type": "org:Organization", "name": "National Institutes of Health"}, "theme": ["NIH"], "title": "Plasma proteins in a standardised skin mini-erosion (I): permeability changes as a function of time"}