{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Narrowe, Adrienne", "hasEmail": "mailto:adrienne.narrowe@usda.gov"}, "description": "<p>In vitro study of the effects on the human gut microbiota of lemon pectins with two different molecular weights and having varying degrees of esterification.  Data collected following incubations include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (found in the NCBI Sequence Read Archive associated with BioProject PRJNA903836: <a href=\"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA903836\">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA903836</a>), RT-qPCR of Bifidobaterium sp. 16S rRNA genes, and short-chain fatty acid concentrations. </p>\n<p>Resources in this dataset:</p>\n<ul>\n<li>\n<p>Resource Title: Short Chain Fatty Acid concentrations\nFile Name: pectin_SCFA_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Pectin_total_bacterial_qPCR_data\nFile Name: pectin_total_bacterial_qPCR_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Pectin_bifidobacterium_genus_qPCR_data\nFile Name: pectin_bifidobacterium_genus_qPCR_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Sample metadata</p>\n</li>\n<li>\n<p>File Name: pectin_sample_attributes.csv</p>\n</li>\n</ul>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43756536", "format": "csv", "mediaType": "text/plain", "title": "pectin_sample_attributes_0.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43756539", "format": "csv", "mediaType": "text/plain", "title": "pectin_SCFA_data_0.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43756542", "format": "csv", "mediaType": "text/plain", "title": "pectin_total_bacterial_qPCR.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43756545", "format": "csv", "mediaType": "text/plain", "title": "pectin_bifidobacteria_genus_qPCR.csv"}], "identifier": "10.15482/USDA.ADC/1529137", "keyword": ["16S rRNA sequence data", "short chain fatty acids", "pectin", "RT-qPCR", "NP306", "data.gov", "ARS"], "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2025-11-21", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "spatial": "{\"type\": \"Point\", \"coordinates\": [-75.187092456102, 40.078126249741]}", "temporal": "2018-10-16/2018-10-16", "title": "Data from: Modulation of the Gut Microbiota Structure and Function by Two Structurally Different Lemon Pectins"}