gov.noaa.nodc:0311431 eng utf8 dataset NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NOAA Contact Information Information for contacts at NCEI. information custodian 2026-02-09T13:16:21Z ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Characterizing the Biological Carbon Pump and Evaluating Impacts of OA on Microbial and Planktonic Communities in the Gulf of America from 2021-12-06 to 2025-05-24 (NCEI Accession 0311431) 2026-01-07 publication 2026-02-03 revision 2026-01-07 creation International DOI Foundation (IDF) 10.25921/0yg2-e015 NOAA National Centers for Environmental Information gov.noaa.nodc:0311431 NCEI Archive Management System 0311431 NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information publisher Gabriella Lirio Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) gabriella.lirio@noaa.gov https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information resourceProvider Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information resourceProvider Luke Thompson Northern Gulf Institute (NGI) luke.thompson@noaa.gov https://www.northerngulfinstitute.org/ HTTPS Northern Gulf Institute (NGI) website Institution web page information principalInvestigator Enrique Montes Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) enrique.montes@noaa.gov https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information principalInvestigator Leticia Barbero NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML), Cooperative Institute for Marine and Atmospheric Studies Leticia.Barbero@noaa.gov principalInvestigator Christopher Moore St. Petersburg Coastal and Marine Science Center, US Geological Survey principalInvestigator Emily Osborne NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) Emily.Osborne@noaa.gov https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information principalInvestigator Caitlin E. Reynolds St. Petersburg Coastal and Marine Science Center, US Geological Survey principalInvestigator Julie Richey St. Petersburg Coastal and Marine Science Center, US Geological Survey principalInvestigator Rik Wanninkhof NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) Rik.Wanninkhof@noaa.gov https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information principalInvestigator Thompson, Luke Northern Gulf Institute (NGI) https://www.northerngulfinstitute.org/ HTTPS Northern Gulf Institute (NGI) website Institution web page information author Barbero, Leticia NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML), Cooperative Institute for Marine and Atmospheric Studies author Lirio, Gabriella Cooperative Institute For Marine And Atmospheric Studies https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information author Montes, Enrique Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information author Moore, Christopher St. Petersburg Coastal and Marine Science Center, US Geological Survey author Osborne, Emily NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author Reynolds, Caitlin E. St. Petersburg Coastal and Marine Science Center, US Geological Survey author Richey, Julie St. Petersburg Coastal and Marine Science Center, US Geological Survey author Wanninkhof, Rik NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) https://www.aoml.noaa.gov/ HTTPS NOAA Atlantic Oceanographic and Meteorological Laboratory (AOML) website Institution web page information author Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) https://cimas.earth.miami.edu/ HTTPS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies (CIMAS) website Institution web page information contributor Northern Gulf Institute (NGI) https://www.northerngulfinstitute.org/ HTTPS Northern Gulf Institute (NGI) website Institution web page information contributor tableDigital This dataset contains the seawater data (dissolved inorganic carbon, total alkalinity, and nutrients) collected from seven Northern Gulf of America Sediment Trap cruises (GMT-1 through 7). GMT-1 took place December 5-7, 2021 aboard the R/V Pelican. GMT-2 took place May 14-16, 2022 aboard the R/V Weatherbird II. GMT-3 took place November 29-December 1, 2022 aboard the R/V Pelican. GMT-4 took place May 27-June 2, 2023 aboard the R/V Weatherbird II. GMT-5 took place November 29-December 1, 2023 aboard the R/V Pelican. GMT-6 took place September 23-25, 2024 aboard the R/V Pelican. GMT-7 took place May 21-27, 2025 aboard the R/V Weatherbird II. In total, 84 bottles were collected on the seven cruises. Each cruise also involved the deployment and/or retrieval of a sediment trap at a depth of 550 meters. The purpose of the seawater and sediment samples was to characterize the biological carbon pump using environmental DNA, particle fluxes, and physical and chemical parameters, to identify OA impacts to microbial and planktonic communities in the Northern Gulf. The work conducted on this cruise was supported by the NOAA Ocean Acidification Program. This dataset is available to the public for a wide variety of uses including scientific research and analysis. Funding Information: NOAA Ocean Acidification Program (Characterizing the Biological Carbon Pump and Evaluating Impacts of OA on Microbial and Planktonic Communities in the Gulf of America) completed historicalArchive NOAA National Centers for Environmental Information ncei.info@noaa.gov https://www.ncei.noaa.gov/contact HTTPS NCEI Contact Information Information for contacts at NCEI. information pointOfContact asNeeded NCEI Accession 0311431 was revised and a new version of the archival package was published. Updates to existing archival packages may provide additional files or replace obsolete files. The latest version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. Please see journal.txt in the /about directory for additional details on changes made. https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0311431 Preview graphic PNG 0311431 NCEI ACCESSION NUMBER 2026-01-26 publication AMMONIUM (NH4) DISSOLVED INORGANIC CARBON (DIC) NITRATE nitrate + nitrite content (concentration) NITRITE pH phosphate SALINITY silicate total alkalinity WATER TEMPERATURE theme NODC DATA TYPES THESAURUS carbon dioxide (CO2) gas analyzer CTD Niskin bottle nutrient autoanalyzer pH sensor salinometer temperature sensor titrator instrument NODC INSTRUMENT TYPES THESAURUS chemical discrete sample profile theme NODC OBSERVATION TYPES THESAURUS R/V Pelican WEATHERBIRD II platform NODC PLATFORM NAMES THESAURUS Northern Gulf Institute Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies dataCentre NODC COLLECTING INSTITUTION NAMES THESAURUS Rosenstiel School of Marine, Atmospheric, and Earth Science Cooperative Institute for Marine and Atmospheric Studies dataCentre NODC SUBMITTING INSTITUTION NAMES THESAURUS Ocean Carbon and Acidification Data System (OCADS) US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP) project NODC PROJECT NAMES THESAURUS Gulf of America Gulf of Mexico place NODC SEA AREA NAMES THESAURUS oceanography theme WMO_CategoryCode 2012-09-15 publication GMT-1 GMT-2 GMT-3 GMT-4 GMT-5 GMT-6 GMT-7 project Cruise ID 320G20220514 320G20230527 320G20250521 32PE20211205 32PE20221129 32PE20231129 32PE20240923 project EXPOCODE Ocean Acidification Program (OAP) Ocean Carbon and Acidification Data System (OCADS) Project project Ocean Acidification Search Keywords Coastal project Reference Section ID EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > AMMONIA EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRITE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PHOSPHATE EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > SILICATE EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY theme Global Change Master Directory (GCMD) Science Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Profile theme OCADS Study Type DIC DIC_AOML N+N NH4 NO3 NO4 PO4 Si TA TA_AOML theme Provider Variable Abbreviations AUTOANALYZER > AUTOANALYZER CO2 ANALYZERS > CO2 ANALYZERS CTD > Conductivity, Temperature, Depth NISKIN BOTTLES PH METERS > PH METERS SALINOMETERS TEMPERATURE SENSORS > TEMPERATURE SENSORS instrument Global Change Master Directory (GCMD) Instrument Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Ships platform Global Change Master Directory (GCMD) Platform Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian PELICAN (call sign: WSK3051, ICES code: 32PE, 1985-) WEATHERBIRD (call sign: AGOR, ICES code: 320G) platform ICES/SeaDataNet Ship Codes OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO place Global Change Master Directory (GCMD) Location Keywords 2026 revision 23 Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA) Greenbelt MD https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords HTTPS GCMD Keyword Forum Page Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords information custodian Gulf of America Gulf of Mexico place Provider Geographic Names accessLevel: Public otherRestrictions Cite as: Thompson, Luke; Barbero, Leticia; Lirio, Gabriella; Montes, Enrique; Moore, Christopher; Osborne, Emily; Reynolds, Caitlin E.; Richey, Julie; Wanninkhof, Rik (2026). Characterizing the Biological Carbon Pump and Evaluating Impacts of OA on Microbial and Planktonic Communities in the Gulf of America from 2021-12-06 to 2025-05-24 (NCEI Accession 0311431). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/0yg2-e015. Accessed [date]. Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives. otherRestrictions Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose. license These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0). SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0) NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system 2022-04 publication https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system HTTPS https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system OCADS website information NOAA National Centers for Environmental Information author NOAA National Centers for Environmental Information (NCEI) publisher documentDigital [accessed 2024-02-08] crossReference program Global Coastal Program Data: North America East Coast 2022 publication https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/north_america_east.html HTTPS https://www.ncei.noaa.gov/access/ocean-carbon-acidification-data-system/oceans/Coastal/north_america_east.html related resource information NOAA National Centers for Environmental Information (NCEI) publisher documentDigital crossReference project eng; USA utf8 environment oceans -89.137 -89.1063 23.2302 23.2398 2021-12-06 2025-05-24 This data package (Submission ID: BJJ1WUNXR) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL. 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Originator data format 0.272 https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0311431.html HTTPS Web browser Project Metadata Navigate directly to the URL for a descriptive web page with download links. information https://doi.org/10.25921/0yg2-e015 HTTPS Web browser NCEI Dataset Landing Page Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/oas/311431 HTTPS Web browser Descriptive Information Navigate directly to the URL for a descriptive web page with download links. information https://www.ncei.noaa.gov/archive/accession/download/311431 HTTPS Web browser HTTPS Navigate directly to the URL for data access and direct download. download ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0240/0311431/ FTP Any FTP client FTP These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP). download dataset NCEI Accession 0311431 v1.1 was published. 2026-01-07T22:06:27Z NCEI Accession 0311431 v1.1 publication https://www.ncei.noaa.gov/archive/accession/0311431/1.1 HTTPS NCEI Accession 0311431 v1.1 published 2026-01-07T22:06:27Z download NCEI Accession 0311431 was revised and v2.2 was published. Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. 2026-01-26T17:07:29Z NCEI Accession 0311431 v2.2 publication https://www.ncei.noaa.gov/archive/accession/0311431/2.2 HTTPS NCEI Accession 0311431 v2.2 published 2026-01-26T17:07:29Z download NCEI Accession 0311431 was revised and v3.3 was published. Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. 2026-02-03T17:36:22Z NCEI Accession 0311431 v3.3 publication https://www.ncei.noaa.gov/archive/accession/0311431/3.3 HTTPS NCEI Accession 0311431 v3.3 published 2026-02-03T17:36:22Z download dataset Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: micro-mol/kg; Observation type: Profile; In-situ / Manipulation / Response variable: IN-SITUOBSERVATION; Measured or calculated: MEASURED; Sampling instrument: Niskin bottle; Analyzing instrument: (USGS) UIC, Inc. carbon coulometer nidek 5017L | (AOML) Two systems consisting of a coulometer (UIC Inc.) coupled with a Dissolved Inorganic Carbon Extractor (DICE) inlet system. DICE was developed by Esa Peltola and Denis Pierrot of NOAA/AOML and Dana Greeley of NOAA/PMEL to modernize a carbon extractor called SOMMA (Johnson et al. 1985, 1987, 1993, and 1999; Johnson 1992); Detailed sampling and analyzing information: (USGS) Detailed sampling and analyzing information: Samples were analyzed for total dissolved inorganic carbon (DIC) with a CM5017 CO 2 carbon coulometer coupled to a CM5330 acidification module (UIC) following the methods described by Dickson and Goyet (1994). Using a 60 mL syringe connected to a 3-way Luer lock valve and stopper to minimize gas exchange, three 20 mL same samples were drawn from each bottle. Sample weights were determined using a Denver Instruments PI-214 analytical balance (± 0.1 mg). Injected sample mass (~20 mL) was determined as the difference in syringe weights before and after each sample was injected through a septum into the stripping chamber of the acidification module. Samples were acidified with 10 mL of 8.5% H 3 PO 4. Analytical grade N 2 was used as the carrier gas for CO 2 from the acidified samples to the coulometer. The titration endpoint was determined by the coulometer, and C T was calculated using software from UIC. | (AOML) Samples for total dissolved inorganic carbon (DIC) measurements were drawn according to procedures outlined in the Guide to best practices for ocean CO2 measurements (Dickson et al., 2007) from Niskin bottles into cleaned 500-ml glass bottles. Bottles were rinsed and filled from the bottom, leaving 6 ml of headspace; care was taken not to entrain any bubbles. After 0.2 ml of saturated HgCl2 solution was added as a preservative, the sample bottles were sealed with glass stoppers lightly covered with Apiezon-L grease and were stored at room temperature to be sent back to the lab for analysis after the cruise. The analysis was done by coulometry with two analytical systems (AOML5 and AOML6) used simultaneously. In the coulometric analysis of DIC, all carbonate species are converted to CO2 (gas) by addition of excess hydrogen ion (acid) to the seawater sample, and the evolved CO2 gas is swept into the titration cell of the coulometer with pure air or compressed nitrogen, where it reacts quantitatively with a proprietary reagent based on ethanolamine to generate hydrogen ions. In this process, the solution changes from blue to colorless, triggering a current through the cell and causing coulometrical generation of OH minus ions at the anode. The OH ions react with the H+, and the solution turns blue again. A beam of light is shone through the solution, and a photometric detector at the opposite side of the cell senses the change in transmission. Once the percent transmission reaches its original value, the coulometric titration is stopped, and the amount of CO2 that enters the cell is determined by integrating the total charge during the titration. The volume of the pipette used to deliver the sample in each system was determined with aliquots of distilled water at known temperature. The weights with the appropriate densities were used to determine the volume of the pipette. Calculation of the amount of CO2 injected was according to the CO2 handbook (Dickson et al., 2007).The instrument has a salinity sensor, but all DIC values were recalculated to a molar weight (micro-mol/kg) using density obtained from the CTD salinity. The DIC values were corrected for dilution by 0.2 ml of saturated HgCl2 used for sample preservation (Measured DIC*1.00037). A correction was also applied for the offset from the CRM. This additive correction was applied for each cell using the CRM value obtained in the beginning of the cell.; Replicate information: (USGS) Duplicate sample analyses were performed on the first sample analyses of the day and on approximately every tenth sample analyzed afterwards during the same day of analysis.; Standardization description: (USGS) Accuracy and precision were determined from the analysis of CRMs from Scripps Institution of Oceanography (Dickson, 2010). | (AOML) The coulometers were calibrated by injecting aliquots of pure CO2 (99.99%) by means of an 8-port valve outfitted with two sample loops with known gas volumes bracketing the amount of CO2 extracted from the water samples for the two AOML systems.; Standardization frequency: (USGS) For each day of sample analysis, standards were analyzed at the beginning and end of the days operations. | (AOML) The stability of each coulometer cell solution was confirmed three different ways: two sets of gas loops were measured at the beginning; also the Certified Reference Material (CRM), supplied by Dr. A. Dickson of UCSD, were measured at the beginning.; CRM manufacturer: (USGS) Scripps Institution of Oceanography of the University of California, San Diego with support from the US National Science Foundation | (AOML) Dr. Andrew Dickson of UCSD; CRM batch number: (USGS) 183, 189, 194, 196, 209 | (AOML) 207, 210; Preservation method: (USGS) Mercuric Chloride (HgCl2) | (AOML) saturated HgCl2; Preservative volume: (USGS) 100 (µl) microliters of 6.5% HgCl 2 | (AOML) 0.2 ml; Preservative correction: (USGS) None | (AOML) The DIC values were corrected for dilution by 0.2 ml of saturated HgCl2 used for sample preservation. The total water volume of the sample bottles was 540 ml (calibrated by Esa Peltola, AOML). The correction factor used for dilution was 1.00037.; Quality flag convention: (USGS) Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performedand element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. | (AOML) WOCE quality control flags are used: 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: (USGS) Yates, K. K., Moore, C. S., Goldstein, N. H., & Sherwood, E. T. (2019). Tampa bay ocean and coastal acidification monitoring quality assurance project plan (No. 2019-1003). US Geological Survey.Dickson, A. G. 2010. Standards for ocean measurements. Oceanography 23: 34–47. doi:10.5670/oceanog.2010.22| (AOML) Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. Johnson, K.M., Kortzinger, A.; Mintrop, L.; Duinker, J.C.; and Wallace, D.W.R. (1999). Coulometric total carbon dioxide analysis for marine studies: Measurement and internal consistency of underway surface TCO2 concentrations. Marine Chemistry 67:123 to 44.Johnson, K.M., Wills, K.D.; Butler, D.B.; Johnson, W.K.; and Wong, C.S. (1993). Coulometric total carbon dioxide analysis for marine studies: Maximizing the performance of an automated gas extraction.Johnson, K.M. (1992). Operator Manual: Single-Operator Multiparameter Metabolic Analyzer (SOMMA) for Total Carbon Dioxide (CT) with Coulometric Detection. Brookhaven National Laboratory, Brookhaven, N.Y. Johnson, K.M.; Williams, P.J.; Brandstrom, L.; and McN. Sieburth, J. (1987). Coulometric total carbon analysis for marine studies: Automation and calibration. Marine Chemistry 21:117 to 33.; Researcher name: (USGS) Christopher Moore | (AOML) Rik Wanninkhof; Researcher institution: U.S. Geological Survey | Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: micro-mol/kg; Observation type: Profile; In-situ / Manipulation / Response variable: IN-SITUOBSERVATION; Measured or calculated: MEASURED; Sampling instrument: Niskin bottle; Analyzing instrument: (USGS) Ocean Optics USB2000 spectrophotometer | (AOML) Semi-automatic titration systems, System 1 consists of a Metrohm 765 Dosimat titrator, a pH meter (Orion 720A, ThermoScientific), a ROSS half cell pH glass electrode (Orion 9101BN, ThermoScientific) and a reference electrode (Orion 900200, ThermoScientific). System 2 consists of a Metrohm 665 Dosimat titrator, a pH meter (Orion 2 star pH Benchtop, ThermoScientific), a ROSS half cell pH glass electrode (Orion 9101BN, ThermoScientific) and a reference electrode (Orion 900200, ThermoScientific).; Type of titration: (USGS) acid titration (HCL) | (AOML) Full Titration; Cell type (open or closed): OPEN; Curve fitting method: (USGS) none | (AOML Least-Square Analysis; Detailed sampling and analyzing information: (USGS) Samples were analyzed for total alkalinity (TA) according to the spectrophotometric methods of Yao and Byrne (1998) and Liu et al. (2015). Water sample mass (~100 g) and masses of added were determined gravimetrically using a Denver Instruments PI-214 analytical balance (± 0.1 mg). Measurements were made in an open top square glass cell connected to an Ocean Optics USB2000 spectrophotometer and LS-1 Tungsten light source where 100 µl of purified bromocresol purple indicator dye (4mM) was administered by pipette and the sample was titrated using 0.100 ± 0.0001 N standardized HCl added with a plastic 10 mL syringe fitted with a Teflon syringe needle. At the end of each titration, the solution was purged of CO2 with a stream of N2 gas that had been pre saturated with Milli-Q H2O. Absorbance measurements were made using the Ocean Optics software package OOIBase 32. The total weight of added acid was determined from the difference in syringe weight before and after acid addition. Solution temperature was determined using a NIST Traceable digital thermometer. Replicate information: Duplicate sample analyses were performed on the first sample analyses of the day and on approximately every tenth sample analyzed afterwards during the same day of analysis. | (AOML) All of the samples were run using leftover water from the same sample bottles used for DIC and pH. Please refer to DIC for detailed information on sampling and conservation of samples. For each measurement, approximately 200 ml of water sample were titrated with an HCl solution with concetrations certified by Dr. Andrew Dickson of UCSD (0.23878 moles per kilogram-solution).; Standardization description: (USGS) Accuracy and precision were determined from the analysis of CRMs from Scripps Institution of Oceanography (Dickson, 2010). | (AOML) 2 CRM samples were run daily on each cell, before and after the seawater samples. The Total Alkalinity for the water samples was corrected using the daily averaged ratios between the certified and measured values of the 2 CRMs run on each cell. This TA titration system has a precision of 0.1 %. All the TA values were directly measured with reference to Certified Reference Material. The accuracy after correction is 0.1%. Please check attached pdf for more details.; Standardization frequency: (USGS) For each day of sample analysis, standards were analyzed at the beginning and end of the days operations. | (AOML) All values were directly measured with reference to Certified Reference Material (Dickson, UCSD). 2 CRM samples were run daily on each cell.; CRM manufacturer: (USGS) Scripps Institution of Oceanography of the University of California, San Diego with support from the US National Science Foundation | (AOML) Dr. Andrew Dickson of UCSD; CRM batch number: (USGS) 183, 189, 194, 196, 209 | (AOML) 207, 210; Preservation method: (USGS) Mercuric Chlorida (HgCl2) | (AOML) saturated HgCl2; Preservative volume: (USGS) 100 (µl) microliters of 6.5% HgCl2 | (AOML) 0.2 ml; Uncertainty: The precision of this method is better than 0.1% and accuracy is 0.1%.; Quality flag convention: (USGS) Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. | (AOML) WOCE quality control flags are used: 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: (USGS) Yates, K. K., Moore, C. S., Goldstein, N. H., & Sherwood, E. T. (2019). Tampa bay ocean and coastal acidification monitoring quality assurance project plan (No. 2019-1003). US Geological Survey.Dickson, A. G. 2010. Standards for ocean measurements. Oceanography 23: 34–47. doi:10.5670/oceanog.2010.22Liu, X., R. H. Byrne, M. Lindemuth, R. Easley, and J. T. Mathis. 2015. An automated procedure for laboratory and shipboard spectrophotometric measurements of seawater alkalinity: Continuously monitored single-step acid additions. Mar. Chem. 174: 141–146. doi:10.1016/j.marchem.2015.06.008Yao, W., and R. H. Byrne. 1998. Simplified seawater alkalinity analysis. Deep-Sea Res. I Oceanogr. Res. Pap. 45: 1383–1392. doi:10.1016/s0967-0637(98)00018-1 | (AOML) Millero, F. J., Zhang, J. Z., Lee, K., & Campbell, D. M. (1993). Titration alkalinity of seawater. Marine Chemistry, 44(2), 153-165.; Researcher name: (USGS) Christopher Moore | (AOML) Rik Wanninkhof; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Silicate; Abbreviation: Si; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Silicate (Si) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. In this method, β-molybdosilicic acid is formed by the reaction of the silicate contained in the sample with molybdate in acidic solution. The β-molybdosilicic acid is then reduced by ascorbic acid to form molybdenum blue. The absorbance of the molybdenum blue, measured at 820 nm, is linearly proportional to the concentration of silicate in the sample. Oxalic acid is introduced to the sample stream before the addition of ascorbic acid to minimize interference from phosphates.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.1574 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zhang, J.-Z., and Berberian, G.A. (1997). Determination of dissolved silicate in estuarine and coastal waters by gas segmented flow colorimetric analysis, U.S. Environmental Protection Agency, (EPA Method 366.0), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Orthophosphate; Abbreviation: PO4; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Orthophosphate (Var2) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. The determination of ortho-phosphate is based on the colorimetric method in which a blue color is formed by the reaction of ortho-phosphate, molybdate and antimony followed by reduction with ascorbic acid at a pH < 1. The reduced blue phospho-molybdenum complex is read at 880 nm.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.0125 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zimmermann, C.F., and C.W. Keefe (1997). Determination of orthophosphate in estuarine and coastal waters by automated colorimetric analysis. U.S. Environmental Protection Agency (EPA method 365.5), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Nitrate; Abbreviation: NO4; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Nitrate (Var3) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. The determination of nitrate and nitrite uses the procedure whereby nitrate is reduced to nitrite at pH 7.5 in a copperized cadmium reduction coil. The nitrite reduced from nitrate plus any nitrite react under acidic conditions with sulfanilamide to form a diazo compound that then couples with N-1-naphthylethylenediamine dihydrochloride (NEDD) to form a reddish-purple azo dye. The absorbance measured at 540 nm is linearly proportional to the concentration of nitrite + nitrate in the sample. Nitrate concentrations are obtained by subtracting nitrite values, which have been separately determined without the cadmium reduction procedure, from the nitrite + nitrate values.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.0263 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zhang, J.-Z., Ortner, P.B. and Fischer, C.J. (1997b). Determination of nitrate and nitrite in estuarine and coastal waters by gas segmented continuous flow colorimetric analysis. U.S. Environmental Protection Agency (EPA Method 353.4), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Nitrite; Abbreviation: NO3; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Nitrite (Var4) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. The determination of nitrite uses the procedure whereby the nitrite ion reacts with sulfanilamide under acidic conditions to form a diazo compound. This compound then couples with N-1-naphthylethylenediamine dihydrochloride to form a reddish-purple azo dye which is measured at 540 nm.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.0096 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zhang, J.-Z., Ortner, P.B. and Fischer, C.J. (1997b). Determination of nitrate and nitrite in estuarine and coastal waters by gas segmented continuous flow colorimetric analysis. U.S. Environmental Protection Agency (EPA Method 353.4), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Nitrate_and_Nitrite; Abbreviation: N+N; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Nitrate_and_Nitrite (Var5) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. The determination of nitrate and nitrite uses the procedure whereby nitrate is reduced to nitrite at pH 7.5 in a copperized cadmium reduction coil. The nitrite reduced from nitrate plus any nitrite react under acidic conditions with sulfanilamide to form a diazo compound that then couples with N-1-naphthylethylenediamine dihydrochloride (NEDD) to form a reddish-purple azo dye. The absorbance measured at 540 nm is linearly proportional to the concentration of nitrite + nitrate in the sample.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.0263 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zhang, J.-Z., Ortner, P.B. and Fischer, C.J. (1997b). Determination of nitrate and nitrite in estuarine and coastal waters by gas segmented continuous flow colorimetric analysis. U.S. Environmental Protection Agency (EPA Method 353.4), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Ammonium; Abbreviation: NH4; Unit: uM or umol/L; Observation type: Profile; In-situ / Manipulation / Response variable: In-situ observation; Measured or calculated: Measured; Sampling instrument: Niskin bottle; Analyzing instrument: Seal Analytical AA500 segmented flow analyzer; Detailed sampling and analyzing information: Samples for Ammonium (Var6) measurements were drawn from Niskin bottles into rinsed 500 mL brown Nalgene bottles. A 60-mL syringe and 0.45 µm membrane were rinsed with sample water before rinsing and filling a 50 mL Falcon tube with filtered seawater. The Falcon tubes were frozen for preservation and transport back to the lab. Samples were thawed for analysis and placed in a sample tray on the AA500. Approximately 5 mL of each sample is drawn into the system. The determination of ammonium is based on the Berthelot reaction whereby ammonia is reacting with phenol and dichloroisocyanuric acid under alkaline conditions to form a blue colored compound which can be measured at 630 nm. A complexing agent is used to prevent the precipitation of calcium and magnesium hydroxides. Sodium nitroprusside is used to enhance the sensitivity.; Replicate information: The number of field duplicates taken is 10% of the total number of samples (i.e. 10 samples require 1 field duplicate).; Uncertainty: The accuracy of values below 0.0327 uM is not guaranteed as this is the minimum detection limit of the instrument.; Quality flag convention: 1 = Sampled but not analyzed; 2 = Acceptable; 3 = Questionable; 4 = Bad; 5 = Not reported; 6 = Average of replicates; 7 = Value below MDL; 9 = Not Sampled; Method reference: Zhang, J.-Z., Ortner, P.B., Fischer, C.J and Moore, L.D. (1997a). Determination of ammonia in estuarine and coastal waters by gas segmented continuous flow colorimetric analysis. U.S. Environmental Protection Agency (EPA Method 349.0), EPA-600-R-97-072.; Researcher name: Enrique Montes; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Dissolved Inorganic Carbon - AOML; Abbreviation: DIC_AOML; Unit: micro-mol/kg; Observation type: Profile; In-situ / Manipulation / Response variable: IN-SITUOBSERVATION; Measured or calculated: MEASURED; Sampling instrument: Niskin bottle; Analyzing instrument: (USGS) UIC, Inc. carbon coulometer nidek 5017L | (AOML) Two systems consisting of a coulometer (UIC Inc.) coupled with a Dissolved Inorganic Carbon Extractor (DICE) inlet system. DICE was developed by Esa Peltola and Denis Pierrot of NOAA/AOML and Dana Greeley of NOAA/PMEL to modernize a carbon extractor called SOMMA (Johnson et al. 1985, 1987, 1993, and 1999; Johnson 1992); Detailed sampling and analyzing information: (USGS) Detailed sampling and analyzing information: Samples were analyzed for total dissolved inorganic carbon (DIC) with a CM5017 CO 2 carbon coulometer coupled to a CM5330 acidification module (UIC) following the methods described by Dickson and Goyet (1994). Using a 60 mL syringe connected to a 3-way Luer lock valve and stopper to minimize gas exchange, three 20 mL same samples were drawn from each bottle. Sample weights were determined using a Denver Instruments PI-214 analytical balance (± 0.1 mg). Injected sample mass (~20 mL) was determined as the difference in syringe weights before and after each sample was injected through a septum into the stripping chamber of the acidification module. Samples were acidified with 10 mL of 8.5% H 3 PO 4. Analytical grade N 2 was used as the carrier gas for CO 2 from the acidified samples to the coulometer. The titration endpoint was determined by the coulometer, and C T was calculated using software from UIC. | (AOML) Samples for total dissolved inorganic carbon (DIC) measurements were drawn according to procedures outlined in the Guide to best practices for ocean CO2 measurements (Dickson et al., 2007) from Niskin bottles into cleaned 500-ml glass bottles. Bottles were rinsed and filled from the bottom, leaving 6 ml of headspace; care was taken not to entrain any bubbles. After 0.2 ml of saturated HgCl2 solution was added as a preservative, the sample bottles were sealed with glass stoppers lightly covered with Apiezon-L grease and were stored at room temperature to be sent back to the lab for analysis after the cruise. The analysis was done by coulometry with two analytical systems (AOML5 and AOML6) used simultaneously. In the coulometric analysis of DIC, all carbonate species are converted to CO2 (gas) by addition of excess hydrogen ion (acid) to the seawater sample, and the evolved CO2 gas is swept into the titration cell of the coulometer with pure air or compressed nitrogen, where it reacts quantitatively with a proprietary reagent based on ethanolamine to generate hydrogen ions. In this process, the solution changes from blue to colorless, triggering a current through the cell and causing coulometrical generation of OH minus ions at the anode. The OH ions react with the H+, and the solution turns blue again. A beam of light is shone through the solution, and a photometric detector at the opposite side of the cell senses the change in transmission. Once the percent transmission reaches its original value, the coulometric titration is stopped, and the amount of CO2 that enters the cell is determined by integrating the total charge during the titration. The volume of the pipette used to deliver the sample in each system was determined with aliquots of distilled water at known temperature. The weights with the appropriate densities were used to determine the volume of the pipette. Calculation of the amount of CO2 injected was according to the CO2 handbook (Dickson et al., 2007).The instrument has a salinity sensor, but all DIC values were recalculated to a molar weight (micro-mol/kg) using density obtained from the CTD salinity. The DIC values were corrected for dilution by 0.2 ml of saturated HgCl2 used for sample preservation (Measured DIC*1.00037). A correction was also applied for the offset from the CRM. This additive correction was applied for each cell using the CRM value obtained in the beginning of the cell.; Replicate information: (USGS) Duplicate sample analyses were performed on the first sample analyses of the day and on approximately every tenth sample analyzed afterwards during the same day of analysis.; Quality flag convention: (USGS) Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performedand element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. | (AOML) WOCE quality control flags are used: 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: (USGS) Yates, K. K., Moore, C. S., Goldstein, N. H., & Sherwood, E. T. (2019). Tampa bay ocean and coastal acidification monitoring quality assurance project plan (No. 2019-1003). US Geological Survey.Dickson, A. G. 2010. Standards for ocean measurements. Oceanography 23: 34–47. doi:10.5670/oceanog.2010.22| (AOML) Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp. Johnson, K.M., Kortzinger, A.; Mintrop, L.; Duinker, J.C.; and Wallace, D.W.R. (1999). Coulometric total carbon dioxide analysis for marine studies: Measurement and internal consistency of underway surface TCO2 concentrations. Marine Chemistry 67:123 to 44.Johnson, K.M., Wills, K.D.; Butler, D.B.; Johnson, W.K.; and Wong, C.S. (1993). Coulometric total carbon dioxide analysis for marine studies: Maximizing the performance of an automated gas extraction.Johnson, K.M. (1992). Operator Manual: Single-Operator Multiparameter Metabolic Analyzer (SOMMA) for Total Carbon Dioxide (CT) with Coulometric Detection. Brookhaven National Laboratory, Brookhaven, N.Y. Johnson, K.M.; Williams, P.J.; Brandstrom, L.; and McN. Sieburth, J. (1987). Coulometric total carbon analysis for marine studies: Automation and calibration. Marine Chemistry 21:117 to 33.; Researcher name: (USGS) Christopher Moore | (AOML) Rik Wanninkhof; Researcher institution: U.S. Geological Survey | Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) Parameter or Variable: Total Alkalinity - AOML; Abbreviation: TA_AOML; Unit: micro-mol/kg; Observation type: Profile; In-situ / Manipulation / Response variable: IN-SITUOBSERVATION; Measured or calculated: MEASURED; Sampling instrument: Niskin bottle; Analyzing instrument: (USGS) Ocean Optics USB2000 spectrophotometer | (AOML) Semi-automatic titration systems, System 1 consists of a Metrohm 765 Dosimat titrator, a pH meter (Orion 720A, ThermoScientific), a ROSS half cell pH glass electrode (Orion 9101BN, ThermoScientific) and a reference electrode (Orion 900200, ThermoScientific). System 2 consists of a Metrohm 665 Dosimat titrator, a pH meter (Orion 2 star pH Benchtop, ThermoScientific), a ROSS half cell pH glass electrode (Orion 9101BN, ThermoScientific) and a reference electrode (Orion 900200, ThermoScientific).; Detailed sampling and analyzing information: (USGS) Samples were analyzed for total alkalinity (TA) according to the spectrophotometric methods of Yao and Byrne (1998) and Liu et al. (2015). Water sample mass (~100 g) and masses of added were determined gravimetrically using a Denver Instruments PI-214 analytical balance (± 0.1 mg). Measurements were made in an open top square glass cell connected to an Ocean Optics USB2000 spectrophotometer and LS-1 Tungsten light source where 100 µl of purified bromocresol purple indicator dye (4mM) was administered by pipette and the sample was titrated using 0.100 ± 0.0001 N standardized HCl added with a plastic 10 mL syringe fitted with a Teflon syringe needle. At the end of each titration, the solution was purged of CO2 with a stream of N2 gas that had been pre saturated with Milli-Q H2O. Absorbance measurements were made using the Ocean Optics software package OOIBase 32. The total weight of added acid was determined from the difference in syringe weight before and after acid addition. Solution temperature was determined using a NIST Traceable digital thermometer. Replicate information: Duplicate sample analyses were performed on the first sample analyses of the day and on approximately every tenth sample analyzed afterwards during the same day of analysis. | (AOML) All of the samples were run using leftover water from the same sample bottles used for DIC and pH. Please refer to DIC for detailed information on sampling and conservation of samples. For each measurement, approximately 200 ml of water sample were titrated with an HCl solution with concetrations certified by Dr. Andrew Dickson of UCSD (0.23878 moles per kilogram-solution).; Uncertainty: The precision of this method is better than 0.1% and accuracy is 0.1%.; Quality flag convention: (USGS) Flag 0= No quality control (QC) performed, Flag 1 = QC has been performed and element appears to be correct, Flag 2 = QC has been performed and element appears to be probably good with other elements, Flag 3 = QC has been performed and element appears to be probably bad, Flag 4 = QC has been performed and element appears to be bad, Flag 5 = the value has been modified as a result of QC, Flag 9 = The value of the element is missing. | (AOML) WOCE quality control flags are used: 2 = good value, 3 = questionable value, 4 = bad value, 5 = value not reported, 6 = mean of replicate measurements, 9 = sample not drawn.; Method reference: (USGS) Yates, K. K., Moore, C. S., Goldstein, N. H., & Sherwood, E. T. (2019). Tampa bay ocean and coastal acidification monitoring quality assurance project plan (No. 2019-1003). US Geological Survey.Dickson, A. G. 2010. Standards for ocean measurements. Oceanography 23: 34–47. doi:10.5670/oceanog.2010.22Liu, X., R. H. Byrne, M. Lindemuth, R. Easley, and J. T. Mathis. 2015. An automated procedure for laboratory and shipboard spectrophotometric measurements of seawater alkalinity: Continuously monitored single-step acid additions. Mar. Chem. 174: 141–146. doi:10.1016/j.marchem.2015.06.008Yao, W., and R. H. Byrne. 1998. Simplified seawater alkalinity analysis. Deep-Sea Res. I Oceanogr. Res. Pap. 45: 1383–1392. doi:10.1016/s0967-0637(98)00018-1 | (AOML) Millero, F. J., Zhang, J. Z., Lee, K., & Campbell, D. M. (1993). Titration alkalinity of seawater. Marine Chemistry, 44(2), 153-165.; Researcher name: (USGS) Christopher Moore | (AOML) Rik Wanninkhof; Researcher institution: Atlantic Oceanographic and Meteorological Laboratory, National Oceanic and Atmospheric Administration (NOAA) asNeeded Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness. NOAA National Centers for Environmental Information custodian carbon dioxide (CO2) gas analyzer carbon dioxide (CO2) gas analyzer measures gaseous carbon dioxide Carbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption. Carbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water. To measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water. CTD CTD Conductivity-Temperature-Depth probe Niskin bottle Niskin bottle Device for obtaining samples of seawater at a specific depth Why special entry for Niskin bottle but no entry for Nansen bottle? nutrient autoanalyzer nutrient autoanalyzer Laboratory Instrument Nutrient Autoanalyzer is a generic term describing an automated flow-thru system used to conduct analyses of several different nutrients (nitrate, ammonium, orthophosphate, and silicate) simultaneously from a seawater sample. For example: SEAL AutoAnalyzer 3 HR Continuous Segmented Flow Analyzer pH sensor pH sensor generic meter used to measure pH Any of a class of instruments used to quantify pH in a water or other type of sample. salinometer salinometer generic salinometer use salinometer when the instrument type used is unclear but the data include salinity measurements temperature sensor temperature sensor A sensor used to measure temperature. titrator titrator titrator R/V Pelican Flag: United States Title: R/V Vessel Type: Research Survey IMO: 8517009 DOB: 198505 Institution: LUMCON/UNOLS WEATHERBIRD II Flag: United States of America Vessel Type: Research Vessel IMO: DOB: 198201 Institution: Bermuda Biological Station for Research Inc Former Names: WEATHERBIRD (1989); AUNT BEA Citations: 1. Lloyd's Register of Ships 2006-2007; 2. The Universal Licensing System of the U.S. Federal Communications Commission