{"@type": "dcat:Dataset", "accessLevel": "public", "bureauCode": ["005:18"], "contactPoint": {"fn": "Narrowe, Adrienne", "hasEmail": "mailto:adrienne.narrowe@usda.gov"}, "description": "<p>The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: <a href=\"https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645\">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645</a>), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. </p>\n<p>Resources in this dataset:</p>\n<ul>\n<li>\n<p>Resource Title: lactose sample attributes\nFile Name: lactose_sample_metadata.csv</p>\n</li>\n<li>\n<p>Resource Title: lactose SCFA, pH, and gas production data\nFile Name: lactose_SCFA_data.csv</p>\n</li>\n<li>\n<p>Resource Title: Total bacterial and Bifidobacterium genus qPCR\nFile Name: lactose_qPCR_all_bacteria_and_bifido.csv</p>\n</li>\n</ul>", "distribution": [{"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43734918", "format": "csv", "mediaType": "text/plain", "title": "lactose_sample_metadata.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43734921", "format": "csv", "mediaType": "text/plain", "title": "lactose_SCFA_data.csv"}, {"@type": "dcat:Distribution", "downloadURL": "https://ndownloader.figshare.com/files/43734930", "format": "csv", "mediaType": "text/plain", "title": "lactose_qPCR_all_bacteria_and_bifido.csv"}], "identifier": "10.15482/USDA.ADC/1529150", "keyword": ["16S rRNA sequence data", "short chain fatty acids", "lactose", "NP306", "data.gov", "ARS"], "license": "https://creativecommons.org/licenses/by/4.0/", "modified": "2025-11-21", "programCode": ["005:040"], "publisher": {"@type": "org:Organization", "name": "Agricultural Research Service"}, "spatial": "{\"type\": \"Point\", \"coordinates\": [-75.187092456102, 40.078126249741]}", "temporal": "2021-06-01/2021-06-01", "title": "Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner"}