The Wyoming Landscape Conservation Initiative Literature Database is a collection of publication records that reference the Wyoming Landscape Conservation Initiative (WLCI). The database was developed in an effort to provide the WLCI community with a more functional publication citation index and storage system. By maintaining the literature database, the WLCI community has the opportunity to streamline their workflow and gain further insight into the work that has been published on in regards to the WLCI effort. The xDD API, ScienceBase Catalog, USGS Publications Warehouse, ORCiD website, USGS Staff Profiles website, WLCI website, and the homepages of WLCI scientists were used to search and compile records. The records were entered into a shared library using Zotero, a reference management software. Zotero, along with other software options like Mendeley, RefWorks, EndNote, etc., facilitate the creation of literature databases. In order to confirm each record in the literature database represented a WLCI publication, each publication was searched for mention of the WLCI. To ensure each record included adequate information, records were automatically cross-referenced using the Zotero software and common digital identifiers (DOI or ISSN) or information was added manually. To improve the database, the Zotero duplication tool was used to detect and merge duplicate records and specific tags were incorporated into each record to indicate where the WLCI was referenced in a publication, how the WLCI was referenced in a publication, and/or through which search method the publication was found. The WLCI library can be accessed through three ways; by downloading the Zotero desktop application and syncing it to the online WLCI library, by accessing the WLCI library in the online Zotero application, and through the Zotero API. The library is open to the public. However, the records can only be added or edited by WLCI community members. Because the database is hosted by a third party provider, this data release offers access to the database records in the form of a CSV and RDF file.