The United States Geological Survey’s (USGS) SPAtially Referenced Regressions On Watershed attributes (SPARROW) model was developed to aid in the interpretation of monitoring data and simulate water-quality conditions in streams across large spatial scales. SPARROW is a hybrid empirical/process-based mass balance model that can be used to estimate the major sources and environmental factors that affect the long-term supply, transport, and fate of contaminants in streams. The spatially explicit model structure is defined by a river reach network coupled with contributing catchments. The model is calibrated by statistically relating watershed sources and transport-related properties to monitoring-based streamflow and water-quality load estimates. Streamflow and load estimates considered for use in regional SPARROW model applications (2012 base year) are described in Saad and others, 2019 (https://dx.doi.org/10.3133/sir20195069). Load estimation methods described in this report include the Beale Ratio Estimator and Fluxmaster models. This USGS data release contains all of the input and output files necessary to reproduce the load estimates considered for inclusion in the 2012 regional SPARROW models. Data preparation for input to the load estimation models is also fully described in the above-mentioned report.