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Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols

Metadata Updated: March 14, 2025

This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices.

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

Downloads & Resources

Dates

Metadata Created Date March 18, 2023
Metadata Updated Date March 14, 2025
Data Update Frequency irregular

Metadata Source

Harvested from NIST

Additional Metadata

Resource Type Dataset
Metadata Created Date March 18, 2023
Metadata Updated Date March 14, 2025
Publisher National Institute of Standards and Technology
Maintainer
Identifier ark:/88434/mds2-2939
Data First Published 2023-03-02
Language en
Data Last Modified 2023-02-03 00:00:00
Category Bioscience:Microbial measurements
Public Access Level public
Data Update Frequency irregular
Bureau Code 006:55
Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id ef6c343d-3a5a-45a7-9720-b5782c1c7d40
Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
Harvest Source Title NIST
Homepage URL https://data.nist.gov/od/id/mds2-2939
License https://www.nist.gov/open/license
Program Code 006:045
Source Datajson Identifier True
Source Hash 0c8cb0c6f9141afadb45afbb4172bedc2451411c1cb75c93c74cdd3fdca02e52
Source Schema Version 1.1

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