In freshwater bodies of New Hampshire, the most problematic aquatic invasive plant species is Myriophyllum heterophyllum or variable leaf water-milfoil. Once established, variable leaf water-milfoil forms dense beds that can alter the limnologic characteristics of a waterbody, impacting natural lacustrine communities and their habitats. Variable leaf water-milfoil infestations also disrupt recreational uses of waterbodies and have negatively affected swimming, boating, fishing, and property values in and around several lakes and ponds in New Hampshire. In 1965, Moultonborough Bay, Lake Winnipesaukee became the first waterbody in New Hampshire where variable leaf water-milfoil was observed. Variable leaf water-milfoil is native to the Southeastern and Midwestern areas of the United States where more alkaline waters appear to limit the growth of this plant. Outside its native range, however, it adapts well to the relatively acidic, low-alkalinity, and nutrient-poor conditions of oligotrophic lakes and bays similar to Moultonborough Bay. In 2005, the New Hampshire Department of Environmental Services (NHDES) collaborated with the U.S. Geological Survey to investigate the distribution (presence and density) of variable leaf water-milfoil in Moultonborough Bay. This study utilized geophysical systems and conventional water-quality measurements to identify lake-floor environments that may provide suitable habitat for the establishment and growth of variable leaf water-milfoil. The results of the study are intended to assist resource managers in federal and state agencies by providing methods for detecting variable leaf water-milfoil and for identifying areas susceptible to infestation. Ultimately, this information may lead to early detection, prevention, and more effective mitigation strategies. Field activity information for this cruise is available on-line through the U.S. Geological Survey Coastal and Marine Geoscience Data System https://cmgds.marine.usgs.gov/fan_info.php?fa=2005-004-FA.