As part of the U.S. Geological Survey's National Water-Quality Assessment Program, an investigation of the Yellowstone River Basin study unit is being conducted to document status and trends in surface- and ground-water quality. Surface-water samples are collected from streams or lakes at specific sampling stations. Efficient quantification of the site-specific geographic and environmental characteristics at sampling stations requires a geospatial data set composed of the site locations that may be processed, together with other digital thematic maps (such as geology or land use), in a geographic information system (GIS). Digital data for 24 stream-sampling stations in the Yellowstone River Basin are included in this data release. The station locations were retrieved from the U.S. Geological Survey's National Water Information System where they are stored as latitude-longitude coordinate pairs referenced to the NAD27 datum. Selected station identifiers and geographic codes also were retrieved and are included in the data release.