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    • Single-molecule long-read methylation profiling reveals regional DNA methylation regulated by Elongator Complex Subunit 2 in Arabidopsis roots experiencing spaceflight

    Metadata Updated: April 10, 2026

    The Advanced Plant Experiment-04 - Epigenetic Expression (APEX-04-EpEx) experiment onboard the International Space Station examined the spaceflight-altered cytosine methylation in two genetic lines of Arabidopsis thaliana, wild-type Col-0 and the mutant elp2-5, which is deficient in an epigenetic regulator Elongator Complex Subunit 2 (ELP2). Whole-genome bisulfite sequencing (WGBS) revealed distinct spaceflight associated methylation differences, presenting the need to explore specific space-altered methylation at single-molecule resolution to associate specific changes over large regions of spaceflight related genes. To date, tools of multiplexed targeted DNA methylation sequencing remain limited for plant genomes. This data set includes single-molecule profiling in user-defined targets using Flap-Enabled Next-Generation Capture (FENGC) on Arabidopsis root tissues to reveal precise modification of DNA methylation regulated by Elongator Complex Subunit 2 during spaceflight.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

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    Dates

    Metadata Created Date April 11, 2025
    Metadata Updated Date April 10, 2026

    Metadata Source

    Harvested from NASA Data.json

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date April 11, 2025
    Metadata Updated Date April 10, 2026
    Publisher Open Science Data Repository
    Maintainer
    Identifier 10.26030/rp5n-0d70
    Data Last Modified 2026-04-06
    Category Biological and Physical Sciences
    Public Access Level public
    Bureau Code 026:00
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 7d757e16-da0e-4ebf-b73c-8b6a86379a88
    Harvest Source Id 58f92550-7a01-4f00-b1b2-8dc953bd598f
    Harvest Source Title NASA Data.json
    License https://www.usa.gov/government-works
    Program Code 026:000
    Source Datajson Identifier True
    Source Hash 9c172aa67a3f72a1cc5c5c262ced16a529a6589f66fdbe31ca5e48a2ffee08ac
    Source Schema Version 1.1

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