This data release contains a set of permeameter test data collected in February and March of 2014 on the Cedar River near Cedar Rapids, Iowa. The permeameter tests were performed in proximity to five horizontal collector wells (HCs) 1, 2, 3, 4, and 6, and three vertical wells that are operated by the City of Cedar Rapids. Each dataset represents groundwater levels over time while individual HC and a few selected vertical wells (S10, S18, S23) had pumps turned on or off to look at the response of the streambed sediments as it relates to the operations of HCs and vertical wells. Three permeameter test locations at the HC2 site and four at the HC1, 3, 4, and 6 were selected across the Cedar River for permeability tests. At each permeameter test location, a hole was drilled through the ice, and a 2-inch clear tube was inserted into the streambed so that the bottom of the tube was filled with streambed sediments and the resulting head after equilibration was representative of the groundwater head. Then, water was poured into the clear tube to measure the groundwater head using submersible pressure transducers recording at 5 second intervals. It was assumed that the water level of the stream was constant during the permeameter test (Chen, 2000). This data release contains the raw water level data in an Excel sheet (PermTestsCalcsAll2014.xlsx), a file (Readme_CR_PermeameterTestData.txt) that provides details about all the files, and an Environmental Systems Research Instituite (ESRI) shapefile (.shp) and its associated files. Reference: Chen, X., 2000, Measurement of streambed hydraulic conductivity and its anisotropy: Environmental Geology, v. 39, p. 1317-1324. [Also available at https://doi.org/10.1007/s002540000172.]