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    • Oyster Larval Survival Counts from Probiotic OY15 Experiments

    Metadata Updated: March 14, 2026

    Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date March 4, 2026
    Metadata Created Date October 19, 2024
    Metadata Updated Date March 14, 2026
    Reference Date(s) 2011 (publication)
    Frequency Of Update asNeeded

    Metadata Source

    Harvested from NMFS NEFSC

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Date March 4, 2026
    Metadata Created Date October 19, 2024
    Metadata Updated Date March 14, 2026
    Reference Date(s) 2011 (publication)
    Responsible Party (Point of Contact, Custodian)
    Contact Email
    Guid gov.noaa.nmfs.inport:26979
    Access Constraints Cite As: Northeast Fisheries Science Center, [Date of Access]: Oyster Larval Survival Counts from Probiotic OY15 Experiments [Data Date Range], https://www.fisheries.noaa.gov/inport/item/26979., Access Constraints: The Milford laboratory is currently involved in a CRADA (Cooperative Research and Development Agreement) with Envera LLC, to mass-culture Milford probiotic strain OY15 and make freeze-dried or spray-dried formulations. This may cause a constraint on access to the data as intellectual property, until the CRADA has been completed (June 2016) and agreed upon with collaborators. NEFSC Data Access Constraint: If data is stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access.
    Bbox East Long -73.043572
    Bbox North Lat 41.222161
    Bbox South Lat 41.212161
    Bbox West Long -73.053572
    Coupled Resource
    Frequency Of Update asNeeded
    Harvest Object Id a60fd088-2310-430e-b4fa-f128b26ba5da
    Harvest Source Id 523189fa-8edc-48e1-9ec5-f571e7dd923c
    Harvest Source Title NMFS NEFSC
    Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
    Lineage Fisher's Island oysters spawned at the Milford Laboratory, and larval bioassays run with 4 treatments: no bacteria control, probiotic OY15, pathogen B183 (Vibrio Corallilyticus) and combination treatment with OY15+B183. Bacteria were dosed every other day and larvae subsamples were taken for survival counts.
    Metadata Language eng
    Metadata Type geospatial
    Old Spatial {"type": "Polygon", "coordinates": [[[-73.053572, 41.212161], [-73.043572, 41.212161], [-73.043572, 41.222161], [-73.053572, 41.222161], [-73.053572, 41.212161]]]}
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2006
    Temporal Extent End 2011

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