These data were compiled to study mercury and selenium concentrations in fish species and assemblages in lotic waterbodies across the Upper Colorado River Basin. Data were compiled from State and Federal agencies. This data table contains raw concentration data, as well as standardized concentrations corrected for differences based on sample type (i.e., tissue type), species-specific bioaccumulation rates (Table S1), and fish size (Table S2). The data were used in linear mixed effects models to estimate average mercury and selenium concentration in fish species and in fish assemblages, including fish total length (cm), sampling location (Sub basin name and GPS coordinates), and sampling year (Figures 2,3, and 4 from manuscript).