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Dataset for development and evaluation of a qPCR method modification that mitigates DNA recovery losses for analyses of E. coli fecal indicator bacteria in Great Lakes region recreational waters

Metadata Updated: March 22, 2026

Results previously reported by EPA/ORD on the modified method have led to its adoption into a Standard Operating Procedure for labs performing qPCR analyses for E. coli at recreational beaches in the state of Michigan. This dataset provides further documentation of the performance and applicability of the modified method for recreational beach water testing including data from an extensive survey that can be used to determine when and where unacceptable levels of DNA losses may occur with and without this method modification in the Great Lakes region.

Please see the attached dataset description document for additional information.

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

Downloads & Resources

Dates

Metadata Created Date March 22, 2026
Metadata Updated Date March 22, 2026

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date March 22, 2026
Metadata Updated Date March 22, 2026
Publisher U.S. Environmental Protection Agency
Maintainer
Identifier https://doi.org/10.23719/d-dnd5
Data Last Modified 2025-09-29
Public Access Level public
Bureau Code 020:00
Schema Version https://project-open-data.cio.gov/v1.1/schema
Harvest Object Id 5377cc47-3477-4af5-8ee9-f6bc182862d7
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
License https://pasteur.epa.gov/license/sciencehub-license.html
Program Code 020:000
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency
Source Datajson Identifier True
Source Hash ed434948ff5bfcbee69e3a4aba90da770c259018b9b3089297db33d0a509fa94
Source Schema Version 1.1

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