The goal of this study was to complete a comprehensive evaluation of a low-dose copper treatment for zebra mussel (Dreissena polymorpha Pallas 1771) suppression. The treatment objective was to maintain an average copper concentration of 60 µg/L in waters above the thermocline for 10 consecutive days. The study was conducted in Lake Minnetonka (Hennepin County, Minnesota) and the copper treatment was applied to St. Albans Bay (66.3-ha). Robinson Bay (37.2-ha) was used as an untreated control reference site. Five locations were identified as sampling sites throughout each bay and marked with a buoy. Test animals were held, and samples were collected in the vicinity of these sampling buoys before, during, and after treatment. The datasets included are as follows: Alkalinity and Hardness Benthic Invertebrate Sampling Results Biotic Ligand Model Data Chlorophyll A Copper Data Mortality SCUBA Zebra Mussel Survey Secchi Disk and Light Intensity Data Test Animal Condition Data Thermocline Data Tissue Copper Residue Summary Water Chemistry Zebra Mussel Settlement Zooplankton Sampling Summary